Cover image for RNA silencing : methods and protocols
Title:
RNA silencing : methods and protocols
Author:
Carmichael, Gordon G.
ISBN:
9781588294364
Publication Information:
Totowa, N.J. : Humana Press, c2005.
Physical Description:
xi, 338 s. : res.
Series:
Methods in molecular biology, 309

Methods in molecular biology (Clifton, N.J.) ; v. 309.
Contents:
Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of Drosophila RNA silencing complexes by native gel electrophoresis / John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans / Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei / Christine E. Clayton ... [et al.] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing / M. Florian Mette ... [et al.] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sébastien Wälchli -- Silencing gene expression with dicer-generated siRNA pools / Jason W. Myers and James E. Ferrell Jr. --

Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector based RNAi technology / Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo / Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni ... [et al.] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and John J. Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs / Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elisavet Maniataki, Maria Dels Angels De Planell Saguer, and Zissimos Mourelatos --

Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou ... [et al.] -- High-throughput analysis of microRNA gene expression using sensitive probes / Mouldy Sioud and Øystein Røsok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu ... [et al.].
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