Evolutionary engineering of industrially important microbial phenotypes / Stephen S. Fong -- Improving protein functions by directed evolution / Nikhil U. Nair and Huimin Zhao -- Engineering DNA and RNA regulatory regions through random mutagenesis and screening / Ichiro Matsumura, Sean A. Lynch, and Justin P. Gallivan -- Evolving pathways and genomes for the production of natural and novel compounds / Ethan T. Johnson and Claudia Schmidt-Dannert -- Models predicting optimized strategies for protein evolution / Jonathan J. Silberg and Peter Q. Nguyen -- Low-copy number plasmids as artificial chromosomes / Kristala L. Jones Prather -- Chromosomal engineering strategies / Kenan C. Murphy -- Regulating gene expression through engineered RNA technologies / Maung Nyan Win and Christina D. Smolke -- Tools designed to regulate translational efficiency / Claes Gustafsson -- Metabolic engineering of the secretory processing pathway in eukaryotes / Mohak Mhatre, Maira P. Pellegrini, and Michael J. Betenbaugh -- Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps / Robert J. Conrado, Thomas J. Mansell, and Matthew P. DeLisa --

Practical pathway engineering : demonstration in integrating tools / Sung Kuk Lee and Jay D. Keasling -- Genome-wide technologies : DNA microarrays, phenotypic microarrays, and proteomics / Seh Hee Jang ... [et al.] -- Monitoring and measuring the metabolome / Maria Rowena N. Monton and Tomoyoshi Soga -- Systems biology, genome-scale models, and metabolic engineering / Sang Yup Lee ... [et al.]-- Cell-free systems for metabolic engineering / Kara A. Calhoun and James R. Swartz -- In silico models for metabolic systems engineering / Kumar Selvarajoo ... [et al.] -- GC-MS for metabolic flux analysis / Christophe Wittmann -- Tools for measuring intermediate and product formation / Marco Oldiges -- Use of AEX-HPLC-ESI-MS for 13C-labeling based metabolic flux analysis in Saccharomyces cerevisiae and Penicillium chrysogenum / Wouter A. van Winden ... [et al.] -- Energy and cofactor issues in fermentation and oxyfunctionalization processes / Bruno B¿hler... [et al.] -- Microbial biosynthesis of fine chemicals : an emerging technology / Zachary L. Fowler and Mattheos Koffas -- Applications of metabolic engineering for natural drug discovery / Yi Tang, Suzanne Ma, and Wladyslaw A. Wojcicki -- Metabolic engineering for alternative fuels / Yandi Dharmadi and Ramon Gonzalez.