Preface -- Significance of Single Cell Sequencing in Future Medicine -- Uncovering Phenotypes with Supercells: Applications to Single-Cell Sequencing -- Single-Cell Sequencing of Microorganisms -- Application of single-cell sequencing in dynamic transcriptomes -- System Immunology in Metabolic Research of Dendritic Cells -- FOXP3+ Treg Cells and Systems Biology Approaches to Studying Their Function -- γδT Cells and αβT Cells -- Prolymphocyte: Lymphocytes and Bioinformatics -- Lymphocytes in liver cancer -- Early biomarkers of hepatocyte necrosis -- Protocol of Single Cell Isolation by Flow Cytometry -- Single-cell based omics-the new era of human disease and medicine -- Cancer bioinformatics.         .