chapter 1 Gene Silencing by RNA Interference and the Role of Small Interfering RNAs -- chapter 2 Basics of siRNA Design and Chemical Synthesis -- chapter 3 Oligonucleotide Scanning Arrays in the Design of Small Interfering RNAs -- chapter 4 siRNA Production by In Vitro Transcription -- chapter 5 Production of siRNAs with the Application of Deoxyribozymes -- chapter 6 Production of siRNA In Vitro by Enzymatic Digestion of Double-Stranded RNA -- chapter 7 Plasmid-Mediated Intracellular Expression of siRNAs -- chapter 8 Lentiviral Vector-Mediated Delivery of si/shRNA -- chapter 9 Exogenous siRNA Delivery: Protocols for Optimizing Delivery to Cells -- chapter 10 RNAi in Drosophila Cell Cultures -- chapter 11 RNAi in Caenorhabditis Elegans -- chapter 12 Delivery of RNAi Reagents in C. elegans by Microinjection -- chapter 13 RNAi in Drosophila Embryos -- chapter 14 RNAi in Xenopus Laevis Oocytes -- chapter 15 RNAi in Xenopus Embryos -- chapter 16 An RNAi-Based Genomic Library for Forward Genetics in the African Trypanosome -- chapter 17 RNAi in the Malaria Parasite Plasmodium -- chapter 18 Silencing the Expression of Cysteine Proteases (Falcipains) in Plasmodium Falciparum using RNA Interference Approaches -- chapter 19 RNAi in Transgenic Animal Models -- chapter 20 RNA Interference in Mouse Models -- chapter 21 Virus-Induced RNA Silencing in Plants -- chapter 22 RNA Silencing in Plants Biolistic Delivery of RNAi Reagents -- chapter 23 Agro-Infiltration: A Versatile Tool for RNAi Studies in Plants.