chapter 1 Strategies for Novel Research Projects -- chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR -- chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries -- chapter 4 Subcloning of Genes or DNA Fragments -- chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing -- chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins -- chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization -- chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems -- chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies -- chapter 10 Analysis of Gene Expression at the Functional Genomic Level -- chapter 11 Analysis of Gene Expression at the Proteomic Level -- chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH) -- chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH) -- chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs -- chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries -- chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression -- chapter 17 New Strategies for Gene Knockout -- chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells -- chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR -- chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology�DNA Microarrays (Gene Chips) -- chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology�Phage Display -- chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology.