chapter 1 Release of Protein from Biological Host / chapter 2 Chromatography: The High-Resolution Technique for Protein Separation / chapter 3 Selectivity in Affinity Chromatography / chapter 4 Genetic Approaches to Facilitate Protein Purification / chapter 5 Solid-Liquid Separation / chapter 6 Membranes for Protein Isolation and Purification / chapter 7 Precipitation of Proteins: Nonspecific and Specific / chapter 8 Protein Crystallization for Large-Scale Bioseparation / chapter 9 Extraction for Rapid Protein Isolation / chapter 10 Adsorption as an Initial Step for the Capture of Proteins / chapter 11 Fast Chromatography of Proteins / chapter 12 Novel Approaches to the Chromatography of Proteins / chapter 13 Scale-up and Commercial Production of Recombinant Proteins / chapter 14 Process Monitoring During Protein Purification / chapter 15 Protein Formulation / chapter 16 Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purification.