This book serves as a comprehensive guide to understanding the complex relationship between digital twins and cybersecurity, providing practical strategies for safeguarding connected systems. This book explores the convergence of digital twins and cybersecurity, offering insights, strategies, and best practices for safeguarding connected systems. It examines the definition, evolution, types, and applications of digital twins across industries like manufacturing, healthcare, and transportation. Highlighting growing digital threats, it underscores the need for robust cybersecurity measures to protect the integrity and confidentiality of digital twin ecosystems. The book analyzes key components and infrastructure of digital twins, including data flow, communication channels, vulnerabilities, and security considerations. It also addresses privacy challenges and explores relevant regulations and compliance requirements. Guiding readers through implementing security measures, it presents a comprehensive cybersecurity framework, covering data protection, encryption, and strategies for ensuring data integrity and confidentiality. It also explores incident response and recovery, secure communication protocols, and the roles of gateways and firewalls. Industry-specific challenges and mitigation strategies are examined through real-world case studies, offering valuable insights and lessons learned. Emerging trends in digital twin technology are thoroughly explored, including the impact of advancements such as AI and quantum computing and their associated cybersecurity challenges and solutions. Audience This book is an essential resource for professionals in the fields of cybersecurity and industrial and infrastructure sectors, including manufacturing, healthcare, transportation, and other industries that utilize digital twins. Researchers in computer science, cybersecurity, engineering, and technology, as well as policymakers and regulatory bodies, will also find this book highly useful.