This book constitutes the refereed proceedings of the International Symposium on Logical Foundations of Computer Science, LFCS 2016, held in Deerfield Beach, FL, USA in January 2016. The 27 revised full papers were carefully reviewed and selected from 46 submissions. The scope of the Symposium is broad and includes constructive mathematics and type theory; homotopy type theory; logic, automata, and automatic structures; computability and randomness; logical foundations of programming; logical aspects of computational complexity; parameterized complexity; logic programming and constraints; automated deduction and interactive theorem proving; logical methods in protocol and program verification; logical methods in program specification and extraction; domain theory logics; logical foundations of database theory; equational logic and term rewriting; lambda and combinatory calculi; categorical logic and topological semantics; linear logic; epistemic and temporal logics; intelligent and multiple-agent system logics; logics of proof and justification; non-monotonic reasoning; logic in game theory and social software; logic of hybrid systems; distributed system logics; mathematical fuzzy logic; system design logics; and other logics in computer science.