Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- 1.1 Preface -- 1.2 Historical Perspective -- 1.3 Sampling and Preanalytics -- 1.4 Reference Materials and Biological Reference Ranges -- 1.5 Clinical Lipidomics -- 1.6 Identification and Annotation -- 1.7 Quantitation -- 1.8 Lipid Ontology -- References -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- 2.1 Safety -- 2.2 Introduction -- 2.3 Sample Origin -- 2.4 Sample Collection -- 2.5 Tissue Homogenization

2.5.1 Mortar and Pestle -- 2.5.2 Rotor-Stator -- 2.5.3 Blender -- 2.5.4 Potter-Elvehjem -- 2.5.5 Bead Mill -- 2.6 Liquid-Liquid Extraction (LLE) -- 2.6.1 Folch Method -- 2.6.2 Bligh and Dyer (BD) Method -- 2.6.3 Modified Folch and Bligh/Dyer (BD) Methods -- 2.6.4 Rose and Oaklander (RO) Method -- 2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method -- 2.6.6 BUME Method -- 2.6.7 Alshehry Method -- 2.6.8 Three-Phase Lipid Extraction (3PLE) -- 2.7 Resuspension and Solubilization -- 2.8 Automation -- 2.9 Tips and Tricks -- Acknowledgments -- References

Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- 3.1 Introduction -- 3.2 Complexity of Crude Lipid Extracts -- 3.2.1 Main Lipid Classes in Mammalian Samples -- 3.2.2 Bond Types as Structural Features -- 3.2.3 Fatty Acids as the Major Building Blocks -- 3.2.4 Lipid Species and Double-Bond Series -- 3.3 Introduction to Mass Spectrometry of Lipids -- 3.3.1 Annotation of Lipid Structures Analyzed by MS -- 3.3.2 Isomers -- 3.3.3 Isobars and the Type-II Isotopic Overlap -- 3.4 Overview of Direct Infusion MS Workflows -- 3.5 Sample Preparation

3.5.1 Preanalytics - Sample Stability -- 3.5.2 Lipid Extraction -- 3.5.3 Solvents, Additives, and Lipid Concentration -- 3.5.4 Sample Derivatization -- 3.6 Direct Infusion -- 3.7 Mass Spectrometry Analysis -- 3.7.1 Electrospray Ionization of Lipids -- 3.7.2 Tandem Mass Spectrometry -- 3.7.3 Multidimensional MS Shotgun Lipidomics -- 3.7.4 High-Resolution Mass Spectrometry -- 3.8 Lipid Identification -- 3.8.1 Identification by MS/MS -- 3.8.2 Identification by HRMS -- 3.8.3 Consideration of Type-II Overlap -- 3.8.4 Identification Hierarchy -- 3.8.5 Caveats/Pitfalls -- 3.9 Lipid Quantification

3.9.1 Internal Standards -- 3.9.2 Type-I Isotopic Effect -- 3.9.3 Evaluation and Correction of Isotopic Overlap -- 3.9.4 Species Response -- 3.9.5 Calculation of Concentration -- 3.10 Data Analysis/Software -- 3.11 Limitations -- 3.12 Selected Applications -- 3.12.1 Analysis of Plasma -- 3.12.2 Analysis of Tissues and Cells -- 3.12.3 Analysis of Lipid Metabolism -- 3.13 Outlook -- References -- Chapter 4 Liquid Chromatography -- and Supercritical Fluid Chromatography -- Mass Spectrometry -- 4.1 Introduction -- 4.2 Lipid Class Separation -- 4.2.1 Normal-Phase Liquid Chromatography