Directed Evolution. Continuous Evolution of Proteins In Vivo / Alon Wellner, Arjun Ravikumar, Chang C Liu -- In Vivo Biosensors for Directed Protein Evolution / Song Buck Tay, Ee Lui Ang -- High-Throughput Mass Spectrometry Complements Protein Engineering / Tong Si, Pu Xue, Kisurb Choe, Huimin Zhao, Jonathan V Sweedler -- Recent Advances in Cell Surface Display Technologies for Directed Protein Evolution / Maryam Raeeszadeh-Sarmazdeh, Wilfred Chen -- Iterative Saturation Mutagenesis for Semi-rational Enzyme Design / Ge Qu, Zhoutong Sun, Manfred T Reetz -- Rational and Semi-Rational Design. Data-driven Protein Engineering / Jonathan Greenhalgh, Apoorv Saraogee, Philip A Romero -- Protein Engineering by Efficient Sequence Space Exploration Through Combination of Directed Evolution and Computational Design Methodologies / Subrata Pramanik, Francisca Contreras, Mehdi D Davari, Ulrich Schwaneberg -- Engineering Artificial Metalloenzymes / Kevin A Harnden, Yajie Wang, Lam Vo, Huimin Zhao, Yi Lu --Engineered Cytochromes P 450 for Biocatalysis / Hanan Alwaseem, Rudi Fasan -- Applications in Industrial Biotechnology. Protein Engineering Using Unnatural Amino Acids / Yang Yu, Xiaohong Liu, Jiangyun Wang -- Application of Engineered Biocatalysts for the Synthesis of Active Pharmaceutical Ingredients (APIs) / Juan Mangas-Sanchez, Sebastian C Cosgrove, Nicholas J Turner -- Directing Evolution of the Fungal Ligninolytic Secretome / Javier Via-Gonzalez, Miguel Alcalde -- Engineering Antibody-Based Therapeutics: Progress and Opportunities / Annalee W Nguyen, Jennifer A Maynard -- Programming Novel Cancer Therapeutics: Design Principles for Chimeric Antigen Receptors / Andrew J Hou, Yvonne Y Chen -- Applications in Medical Biotechnology. Development of Novel Cellular Imaging Tools Using Protein Engineering / Praopim Limsakul, Chi-Wei Man, Qin Peng, Shaoying Lu, Yingxiao Wang.