Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosisof Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. .