Title:
Biological field emission scanning electron microscopy. Volume 1
Author:
Fleck, Roland A., 1970- editor.
ISBN:
9781118663240
9781118663264
9781118663233
Edition:
First edition.
Physical Description:
1 online resource
Series:
RMS-Wiley series.
Contents:
Intro; Table of Contents; About the Editors; List of Contributors; Foreword; Volume I; 1 Scanning Electron Microscopy: Theory, History and Development of the Field Emission Scanning Electron Microscope; 1.1 The Scanning Electron Microscope; 1.2 The Thermionic GUN; 1.3 The Lanthanum Hexaboride ("LaB6") Source; 1.4 Other Enhanced "higher Brightness" Sources; 1.5 The Twenty-first Century SEM; 1.6 The Future for Ion Beam Imaging -- Above and Beyond; References; 2 Akashi Seisakusho Ltd -- SEM Development 1972-1986; 2.1 Introduction; 2.2 TEM Development; 2.3 SEM Development, with TEM Repercussions
2.4 TEM Again, but SEM Lives on2.5 MSM (Mini Scanning Microscope) Table-top Series; 2.6 SIGMA (SS) Series; Acknowledgements; References; 3 Development of FE-SEM Technologies for Life Science Fields; 3.1 Introduction; 3.2 Principle of SEM and Mechanism of Resolution; 3.3 Commercialization of Fe-sem and the Impact of Its Application; 3.4 Development of In-lens Fe-sem And Its Impact; 3.5 Introduction of SEMI In-lens Fe-sem; 3.6 Resolution Improvement by the Deceleration Method; 3.7 Popularization of a Schottky Emission Electron Source and Progress of a Cold FE Electric Gun
3.8 Advent of Truly "Easy to Use" FE-SEM3.9 Closing Remarks; Acknowledgements; References; 4 A History of JEOL Field Emission Scanning Electron Microscopes with Reference to Biological Applications; 4.1 The First Jeol Scanning Electron Microscopes; 4.2 The First Cryo-sem; 4.3 Development of Jeol Field Emission Sems; 4.4 In-lens Field Emission SEM Development; 4.5 Introduction of the JEOL SEMI in-Lens; 4.6 Evolution of the JEOL Semi In-lens FE-SEM; 4.7 Development of Beam Deceleration and the JEOL Energy Filter; 4.8 A Unique Aberration Corrected Fe-sem
4.9 Ongoing Semi In-lens Fe-sem Development4.10 JEOL Introduces the Super Hybrid Lens; 4.11 Development of the JEOL Gentle Beam System; 4.12 Conclusion; References; 5 TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy; 5.1 Historical Introduction; 5.2 Biological Samples in SEM; 5.3 Methods For Imaging Biological Samples; 5.4 Imaging of Sensitive Samples; 5.5 Advanced Fe-sem Techniques in Biology; Conclusions; Acknowledgements; References; 6 FEG-SEM for Large Volume 3D Structural Analysis in Life Sciences; 6.1 Introduction
6.2 High Resolution SEM Imaging at Low Accelerating Voltage6.3 Spot Size; 6.4 Beam Penetration; 6.5 Contrast and Signal-to-noise Ratio; 6.6 Serial Block Face Imaging; 6.7 Challenges of SBFI; 6.8 Recent Advances; 6.9 Multienergy Deconvolution; 6.10 Stability; 6.11 Speed; 6.12 Automated Acquisition, Reconstruction, and Analysis; 6.13 Conclusion; References; 7 ZEISS Scanning Electron Microscopes for Biological Applications; 7.1 Biological Imaging using Zeiss Technology; 7.2 3D Imaging -- Live Happens in 3D; 7.3 Correlative Solutions -- for a Deeper Insight; Acknowledgement; References
Abstract:
"The go-to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope - giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy--specifically, those with a need to understand the application, limitations, and strengths of FEGSEM"--Provided by publisher.
Local Note:
John Wiley and Sons
Electronic Access:
https://onlinelibrary.wiley.com/doi/book/10.1002/9781118663233Copies:
Available:*
Library | Material Type | Item Barcode | Shelf Number | Status | Item Holds |
|---|---|---|---|---|---|
Searching... | E-Book | 594523-1001 | QH212 .S3 B56 2019 | Searching... | Searching... |