Total Chemical Synthesis of Proteins

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Title:

Author:

Brik, Ashraf.

ISBN:

9783527823567

9783527823574

Publication Information:

Newark : John Wiley & Sons, Incorporated, 2021.

Physical Description:

1 online resource (627 pages)

General Note:

4.3 Optimization of Peptide Hydrazide-based Native Chemical Ligation.

Contents:

Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Characterization of Protein Molecules Prepared by Total Chemical Synthesis -- 1.1 Introduction -- 1.2 Chemical Protein Synthesis -- 1.3 Comments on Characterization of Synthetic Protein Molecules -- 1.3.1 Homogeneity -- 1.3.2 Amino Acid Sequence -- 1.3.3 Chemical Analogues -- 1.3.4 Limitations of SPPS -- 1.3.5 Folding as a Purification Step -- 1.4 Summary -- References -- Chapter 2 Automated Fast Flow Peptide Synthesis -- 2.1 Introduction -- 2.2 Results -- 2.2.1 Summary -- 2.2.1.1 Mechanical Principles

2.2.1.2 Chemical Principles -- 2.2.1.3 User Interface Principles -- 2.2.1.4 Data Analysis Method -- 2.2.1.5 Outcome -- 2.2.2 First-generation Automated Fast Flow Peptide Synthesis -- 2.2.2.1 Key Findings -- 2.2.2.2 Design of First-generation AFPS -- 2.2.2.3 Characterization of First-generation AFPS -- 2.2.3 Second-generation Automated Fast Flow Peptide Synthesis -- 2.2.3.1 Key Findings -- 2.2.3.2 Design of Second-generation AFPS -- 2.2.3.3 Characterization and Use of Second-generation AFPS -- 2.2.4 Third-generation Automated Fast Flow Peptide Synthesis -- 2.2.4.1 Key Findings

2.2.4.2 Design of Third-generation AFPS -- 2.2.4.3 Characterization of Third-generation AFPS -- 2.2.4.4 Reagent Stability Study -- 2.2.5 Fourth-generation Automated Fast Flow Peptide Synthesis -- 2.2.5.1 Key Findings -- 2.2.5.2 Effect of Solvent on Fast Flow Synthesis -- 2.2.5.3 Design and Characterization of Fourth-generation AFPS -- 2.3 Conclusions -- Acknowledgments -- References -- Chapter 3 N, S- and N, Se-Acyl Transfer Devices in Protein Synthesis -- 3.1 Introduction -- 3.2 N, S- and N, Se-Acyl Transfer Devices: General Presentation, Reactivity and Statistical Overview of Their Utilization

3.2.1 General Presentation of N, S- and N, Se-Acyl Transfer Devices -- 3.2.2 Relative Reactivity of N, S- and N, Se-Acyl Transfer Devices -- 3.2.3 A Statistical Overview of the Synthetic Use of N, S- and N, Se-Acyl Transfer Devices for Protein Total Chemical Synthesis -- 3.3 Preparation of SEA/SeEAoff and SEAlide Peptides -- 3.3.1 Preparation of SEA and SeEA Peptides -- 3.3.2 Preparation of SEAlide Peptides -- 3.4 Redox-controlled Assembly of Biotinylated NK1 Domain of the Hepatocyte Growth Factor (HGF) Using SEA and SeEA Chemistries

3.5 The Total Chemical Synthesis of GM2-AP Using SEAlide-based Chemistry -- 3.6 Conclusion -- References -- Chapter 4 Chemical Synthesis of Proteins Through Native Chemical Ligation of Peptide Hydrazides -- 4.1 Introduction -- 4.2 Development of Peptide Hydrazide-based Native Chemical Ligation -- 4.2.1 Conversion of Peptide Hydrazide to Peptide Azide -- 4.2.2 Acyl Azide-based Solid-phase Peptide Synthesis -- 4.2.3 Acyl Azide-based Solution-phase Peptide Synthesis -- 4.2.4 The Transesterification of Acyl Azide -- 4.2.5 Development of Peptide Hydrazide-based Native Chemical Ligation

Abstract:

How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.

Local Note:

John Wiley and Sons

Subject Term:

Proteins -- Synthesis.

Protéines -- Synthèse.

Physical & Theoretical.

Chemistry.

SCIENCE.

Proteins -- Synthesis

Genre:

Electronic books.

Added Author: