This book constitutes the proceedings of the 16th Mexican Conference on Pattern Recognition, MCPR 2024, held in Xalapa, Mexico, during June 19-22, 2024, Proceedings. The 36 full papers were carefully reviewed and selected from 68 submissions. The papers are organized in subject areas as follows: Pattern Recognition and Machine Learning Techniques; Computer Vision; Medical Applications of Pattern Recognition; Language Processing and Recognition; Deep Learning and Neural Networks.