This two-volume set LNCS 14859-14860 constitutes the proceedings of the 28th Annual Conference on Medical Image Understanding and Analysis, MIUA 2024, held in Manchester, UK, during July 24-26, 2024. The 59 full papers included in this book were carefully reviewed and selected from 93 submissions. They were organized in topical sections as follows: Part I : Advancement in Brain Imaging; Medical Images and Computational Models; and Digital Pathology, Histology and Microscopic Imaging. Part II : Dental and Bone Imaging; Enhancing Low-Quality Medical Images; Domain Adaptation and Generalisation; and Dermatology, Cardiac Imaging and Other Medical Imaging.