This two-volume set constitutes the refereed post-conference proceedings of the 25th International Conference on Enterprise Information Systems, ICEIS 2023, which was held in Prague, Czech Republic, during April 2023. The 41 full papers and 66 short papers presented were carefully reviewed and selected from 213 submissions. They are organized in topical sections as follows: Part One : Databases and Information Systems Integration; Artificial Intelligence and Decision Support Systems; and Information Systems Analysis and Specification. Part Two : Software Agents and Internet Computing; Human-Computer Interaction; and Enterprise Architecture.