This book constitutes thoroughly refereed and revised selected papers from the 12th Iberoamerican Conference on Applications and Usability of Interactive TV, jAUTI 2023, held in Havana, Cuba, during October 16-17, 2023. The 9 full papers included in this book were carefully reviewed and selected from 25 submissions. They were organized in topical sections as follows: technologies, services and content creation for interactive digital TV; health and socialization through interactive TV; user interfaces, interaction and accessibility.