This book constitutes the refereed proceedings of the 43rd International Conference on Computer Safety, Reliability and Security, SAFECOMP 2024, held in Florence, Italy, in September 2024. The 19 full papers included in this volume were carefully reviewed and selected from 80 submissions. They have been organized in topical sections as follows: Fault Injection and Tolerance; System and Software Safety Assurance; Automated Driving Systems; Security of safety-critical systems; Safety Verification; and Autonomous Systems.