This book focuses on the fascinating topic of tumor microenvironment and dendritic cell immunotherapy. It provides readers with comprehensive insights into this field by offering detailed measurement protocols for assessing the biophysical characteristics of cells, along with bioinformatics analysis and mathematical modeling techniques to explore the initiation of dendritic cell-induced T cell immune responses within the tumor microenvironment. We also delve into the effects of chemical factors, mechanical stimulation, and traditional Chinese medicine on dendritic cells in this specific context. By delving into the intricate interactions between the tumor microenvironment and dendritic cells, readers will gain a profound understanding of tumor progression and the potential of dendritic cells as a therapeutic target. Moreover, the book presents novel perspectives that can pave the way for the development of innovative cancer treatment strategies. .