This open-access textbook provides an in-depth introduction into the CRISPR-cas9 technology and explores its use across the gamut of biological model systems. As the subject has risen from a significant new discovery to a mainstream molecular biology practice, it is essential that students of molecular biology understand the fundamentals behind CRISPR-Cas9 technology and how it may be employed efficiently and ethically in research. This volume, edited by experts in both, molecular biology and undergraduate education, will teach not only the fundamentals of using CRISPR-Cas9, but also how to successfully employ this technology in classroom settings. The book is written for undergraduates and advanced high school classes in the area of molecular biology, genetics, genomics and biological engineering and will provide a perfect tool for undergraduate lecturers to prepare their classes.