This book constitutes the refereed proceedings of the 17th International Conference on Bioinformatics and Computational Biology, BICOB 2025, held in San Francisco, CA, USA, on March 17, 2025. The 18 full papers presented in this book were carefully reviewed and selected from 38 submissions. The papers cover a wide range of bioinformatics and computational biology topics, including bioinformatics algorithms, genomics, machine learning applications in bioinformatics, and medical informatics.