This book constitutes the refereed proceedings of the 23rd International Static Analysis Symposium, SAS 2016, held in Edinburgh, UK, in September 2016. The 21 papers presented in this volume were carefully reviewed and selected from 55 submissions. The contributions cover a variety of multi-disciplinary topics in abstract domains; abstract interpretation; abstract testing; bug detection; data flow analysis; model checking; new applications; program transformation; program verification; security analysis; theoretical frameworks; and type checking.