This book constitutes the refereed proceedings of the International Conference on Applications and Techniques in Information Security, ATIS 2016, held in Cairns, Australia, October 2016. The 10 revised full papers and three short papers presented together with two invited talks were carefully reviewed and selected from 38 submissions. The papers are organized in topical sections on invited speeches; attacks on data security systems; detection of attacks on data security systems; data security; data privacy.