ch. 1. Strategies for novel research projects and/or research grant funding -- ch. 2. Rapid isolation of specific cDNAs or genes by PCR -- ch. 3. Construction and screening of subtracted and complete expression cDNA libraries -- ch. 4. Subcloning of genes or DNA fragments -- ch. 5. Nonisotopic and isotopic DNA or RNA sequencing -- ch. 6. Bioinformation superhighway and computer databases of nucleic acids and proteins -- ch. 7. Characterization of DNA or genes by southern blot hybridization -- ch. 8. Gene overexpression by sense RNA in mammalian systems -- ch. 9. Gene underexpression in cultured cells and animals by antisense DNA and RNA strategies -- ch. 10. Analysis of gene expression at functional genomic level using northern blotting or PCR -- ch. 11. Analysis of gene expression at proteomic level via western blotting -- ch. 12. Analysis of cellular DNA or abundance of mRNA by radioactivity in Situ hybridization -- ch. 13. Localization of DNA and abundance of mRNA by fluorescence in Situ hybridization -- ch. 14. In Situ PCR hybridization of low copy genes and in Situ RT-PCR detection of low abundance mRNAs -- ch. 15. Isolation and characterization of genes from genomic DNA libraries -- ch. 16. Mouse stem cells as a model mammalian cell line for gene expression -- ch. 17. Strategies for gene double knockout -- ch. 18. Large-scale expression and purification of recombinant proteins in cultured cells -- ch. 19. Quantitative analysis of functional genome by real-time RT-PCR -- ch. 20. High-throughput analysis of gene expression by cutting-edge technology : DNA microarrays (gene chips) -- ch. 21. Construction and screening of human antibody libraries using phage display technology -- ch. 22. Down-regulation of gene expression in mammalian systems via siRNA technology -- ch. 23. Strategies for gene cloning, expression, and identification of protein-protein interaction -- ch. 24. Conditional gene knockout -- ch. 25. How to write a research manuscript for publication in an English journal -- ch. 26. How to protect your discovery and invention : patent 101 -- ch. 27. Determination of transgene copy numbers and practical biocalculation.