Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

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Cover image for Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

Title:

Author:

Till, Bradley J. author.

ISBN:

9783319162591

Edition:

1st ed. 2015.

Physical Description:

X, 35 p. 9 illus., 3 illus. in color. online resource.

Contents:

Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. .

Subject Term:

Plant biotechnology.

Biology -- Technique.

Biomaterials.

Nucleic acids.

Biological Techniques.

Nucleic Acid.

Added Author:

Jankowicz-Cieslak, Joanna.

Huynh, Owen A.

Beshir, Mayada M.

Laport, Robert G.