Chapter 1. Microbial Biofilms in Endodontics -- Chapter 2. Update in Root Canal Anatomy of Permanent Teeth Using Microcomputed Tomography -- Chapter 3. Syringe Irrigation: Blending Endodontics and Fluid Dynamics -- Chapter 4. Research on Irrigation: Methods and Models -- Chapter 5. Update of Endodontic Irrigating Solutions -- Chapter 6. Complications of Endodontic Irrigation: Dental, Medical, and Legal -- Chapter 7. The Role of the Patency File in Endodontic Therapy -- Chapter 8. Manual Dynamic Activation (MDA) Technique -- Chapter 9. Apical Negative Pressure: Safety, Efficacy and Efficiency -- Chapter 10. Sonic and Ultrasonic Irrigation -- Chapter 11. Continuous Instrumentation and Irrigation: The Self-Adjusting File (SAF) System -- Chapter 12. Ozone Application in Endodontics -- Chapter 13. Irrigation of the Root Canal System by Laser Activation (LAI): PIPS Photon-Induced Photoacoustic Streaming -- Chapter 14. Photodynamic Therapy for Root Canal Disinfection -- Chapter 15. Local Applications of Antibiotics and Antibiotic-Based Agents in Endodontics -- Chapter 16. Intracanal Medication -- Chapter 17. Disinfection in Nonsurgical Retreatment Cases -- Chapter 18. Irrigation in Regenerative Endodontic Procedures -- Chapter 19. Conclusion and Final Remarks.