Cover -- Title Page -- Copyright Page -- Contents -- Untitled -- Preface -- Chapter 1. Principles of Light Microscopy -- 1.1. Introduction -- 1.2. Principle of image formation -- 1.2.1. Geometric approach -- 1.2.2. Wave approach -- 1.2.3. Image formation under coherent light -- 1.2.4. Microscope resolution (diffraction-limited) -- 1.2.5. Abbe's theory of image formation -- 1.3. Optical sectioning techniques in fluorescence microscopy -- 1.3.1. Wide-field techniques -- 1.3.2. Point-scanning techniques -- 1.4. Conclusion -- 1.5. References

Chapter 2. Contrast-based Label-Free Imaging and Phase Measurement -- 2.1. Introduction, the biological object as an index object -- 2.2. Zernike phase contrast -- 2.2.1. Practical application and limitations -- 2.3. Differential interference contrast -- 2.3.1. Practical application and limitations -- 2.4. Other contrast methods with transparent objects -- 2.5. Measuring phase quantitatively: more than just contrast -- 2.6. Conclusions -- 2.7. References -- Chapter 3. Fluorophores and Labeling Methods for Fluorescence Microscopy -- 3.1. Introduction -- 3.2. Basics of fluorophore photophysics

3.3. Fluorescent proteins -- 3.4. Organic dyes -- 3.5. Conclusion -- 3.6. References -- Chapter 4. Quantitative FRAP and FCS -- 4.1. Life is motion -- 4.2. FRAP -- 4.2.1. Basics of FRAP -- 4.2.2. Quantitative principles of single spot FRAP -- 4.2.3. Analytical expression for principal origins of fluorescence recoveries -- 4.2.4. Variable radii FRAP -- 4.2.5. Quantitative imaging FRAP -- 4.2.6. Fluorescence loss in photobleaching -- 4.2.7. Photoactivation/photoconversion -- 4.3. FCS -- 4.3.1. Basic principle of correlation spectroscopy -- 4.3.2. Mathematics of FCS

4.3.3. Analytical expression for different origins of concentration fluctuations -- 4.3.4. Space and time correlation spectroscopy -- 4.4. Conclusion -- 4.5. References -- Chapter 5. Single-Particle Tracking for Nanoscale Dynamics of Biological Samples -- 5.1. Introduction -- 5.2. Nanoscale localization -- 5.3.Trajectory reconstruction -- 5.4. Nanoscale dynamics -- 5.4.1. Basic properties of Brownian motion -- 5.4.2. Quantification of experimental trajectories -- 5.4.3. Time-variable diffusion -- 5.4.4. Beyond Brownian motion: anomalous diffusion -- 5.5. References

Chapter 6. In Depth Microscopy -- 6.1. Introduction -- 6.2. Confocal microscopy -- 6.2.1. Principle -- 6.2.2. Spinning disk confocal microscopy -- 6.2.3. Limitations -- 6.2.4. Photon reassignment approaches -- the new confocal systems -- 6.3. Multi-photon microscopy -- 6.3.1. Principle -- 6.3.2. Laser-scanning multi-photon microscopy optical setup -- 6.3.3. Widefield multi-photon microscopy by temporal focusing -- 6.4. Light-sheet fluorescence microscopy -- 6.4.1. Principles and advantages -- 6.4.2. Light-sheet creation and properties -- 6.4.3. Main implementation overview