Cover image for The condensed protocols from molecular cloning : a laboratory manual
Title:
The condensed protocols from molecular cloning : a laboratory manual
Author:
Sambrook, Joseph.
ISBN:
9780879697723

9780879697716
Publication Information:
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.
Contents:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
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Book 7.2/12/397734 QH 440.5 S187 2006
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