Part I  Biochemistry and Molecular Biology Toolbox -- Chapter 1 Isolation, Identification and Biochemical Characterization of Azospirillum spp. and other Nitrogen-Fixing Bacteria -- Chapter 2 Molecular Tools for Identification and Characterization of Plant Growth Promoting Rhizobacteria with emphasis in Azospirillum spp -- Chapter 3 In situ Localization and Strain-Specific Quantification of Azospirillum and other Plant Growth Promoting Rhizobacteria Using Antibodies and Molecular Probes -- Chapter 4 Molecular Tools to Study Azospirillum sp. and other Related Plant Growth Promoting Rhizobacteria -- Chapter 5 Genomic Tools for the Study of Azospirillum and other Plant Growth Promoting Rhizobacteria -- Part II Physiology and Metabolism Toolbox -- Chapter 6 Chemotaxis and other Forms of Taxis Behaviors in Azospirillum spp -- Chapter 7 Phytohormones and other Plant Growth Regulators Produced by PGPR: The Genus Azospirillum -- Chapter 8 Inorganic Nitrogen Metabolism in Azospirillum spp -- Chapter 9 Nitric Oxide in Azospirillum and Related Bacteria: Production and Effects -- Chapter 10 Cell Aggregation, Attachment and Plant Interaction -- Chapter 11 Methods for Studying Biofilms in Azospirillum and other Plant Growth Promoting Rhizobacteria -- Chapter 12 Methods for Studying Phenotypic Variation in Azospirillum -- Chapter 13 Polyhydroxybutyrate in Azospirillum brasilense -- Chapter 14 Siderophores Production by Azospirillum: Biological Importance, Assessing methods and Biocontrol activity -- Chapter 15 Cell-Cell Communication in Azospirillum and Related Plant Growth Promoting Rhizobacteria -- Chapter 16 Methods to Study 1-Aminocyclopropane-1-Carboxylate Deaminase in Plant Growth Promoting Bacteria -- Chapter 17 Production of Volatile Organic Compounds in Plant Growth Promoting Rhizobacteria -- Chapter 18 Stress Physiology in Azospirillum and other Plant Growth PromotingRhizobacteria -- Part III Biotic Interactions Toolbox -- Chapter 18 Alleviation of Abiotic and Biotic Stresses in Plants by Azospirillum -- Chapter 19 Interaction of Azospirillum spp. with Microalgae: a Basic Eukaryotic-Prokaryotic Model and its Biotechnological Applications -- Chapter 20 Pseudomonas and Azospirillum -- Chapter 21 Co-inoculation of Legumes with Azospirillum and Symbiotic Rhizobia -- Chapter 22 Interaction of Azospirillum and Mycorrhiza -- Part IV Agronomic and Industrial Applications Toolbox -- Chapter 23 Field Evaluation of Extensive Crops Inoculated with Azospirillum sp -- Chapter 24 Azospirillum spp. and Related Plant Growth Promoting Rhizobacteria Inocula Use in Intensive Agriculture -- Chapter 25 Inoculant Preparation and Formulations for Azospirillum spp -- Chapter 26 Protocol for the Quality Control of Azospirillum spp. Inoculants.