chapter 1 Chapter 1 -- General Principles -- chapter CONTENTS -- chapter 1 1 POLYMERASE CHAIN REACTION (PCR) -- chapter 1 3 IN SITU PCR/RT-PCR -- chapter 1 4 DIRECT IN SITU PCR/RT-PCR -- chapter 2 Chapter 2 -- Preparation of Samples -- chapter CONTENTS -- chapter 3 Chapter 3 -- Pretreatments -- chapter CONTENTS -- chapter 3 3 DEWAXING -- 3.3.1 -- chapter 3 6 POSTFIXATION -- 3.6.1 -- chapter 3 8 DEHYDRATION -- 3.8.1 -- chapter 3 10 RECAPITULATION OF THE CONSEQUENCES OF PRETREATMENTS -- chapter 4 - Reverse Transcription (RT) -- Reverse Transcription -- chapter CONTENTS -- chapter 4 2 DIAGRAM OF THE DIFFERENT STEPS 4.3 THE TOOLS -- 4.3.1 Primers -- chapter 4 4 MATERIALS/REAGENTS/SOLUTIONS -- 4.4.1 Thermocycler -- chapter 5 - Polymerase Chain Reation (PCR) -- Polymerase Chain Reaction (PCR) -- chapter CONTENTS -- chapter 5 2 DIAGRAM OF THE DIFFERENT STEPS -- chapter 5 3 TOOLS -- 5.3.1 Deoxynucleotide Triphosphates (dNTP) -- chapter 6 Chapter 6 -- Hybridization -- chapter CONTENTS -- chapter 6 3 TOOLS: THE PROBES -- chapter 6 4 HYBRIDIZATION PARAMETERS -- chapter 6 7 BEFORE REVELATION -- 6.7.1 Radioacti Hybrids -- chapter 7 Chapter 7 -- Revelation -- chapter CONTENTS -- chapter 8 Chapter 8 -- Electron Microscopy -- chapter CONTENTS -- chapter 8 1 OVERVIEW -- 8.1.1 Objective -- chapter 8 3 SAMPLES -- 8.3.1 Origin -- chapter 8 8 PCR -- 8.8.1 Overview -- chapter 9 Chapter 9 -- Controls and Problems -- chapter CONTENTS -- chapter 9 3 CONTROLS -- 9.3.1 Tools and Reagents -- chapter 10 Chapter 10 -- Typical Protocols -- chapter CONTENTS -- chapter 10 2 INDIRECT IN SITU PCR -- chapter 11 Chapter 11 -- Examples of Observations -- chapter FIGURE11. 1 ( ColorFigure11. 1followspage336. ) -- chapter FIGURE11. 2 ( ColorFigure11. 2followspage336. ) -- chapter FIGURE11. 3 ( ColorFigure11. 3followspage336. ) -- chapter FIGURE11. 4 ( ColorFigure11. 4followspage336. ) -- chapter FIGURE11. 5 ( ColorFigure11. 5followspage336. ) -- chapter FIGURE11. 6 ( ColorFigure11. 6followspage336. ) -- chapter FIGURE11. 7 ( ColorFigure11. 7followspage336. ) -- chapter FIGURE11. 8 ( ColorFigure11. 8followspage336. ) -- chapter FIGURE11. 9 ( ColorFigure11. 9followspage336. ) -- chapter FIGURE11. 1 0 ( ColorFigure11. 10followspage336. ) -- chapter FIGURE11. 1 1 ( ColorFigure11. 11followspage336. ) -- chapter FIGURE11. 1 2 -- chapter FIGURE11. 1 3 -- chapter FIGURE11. 1 4 -- chapter FIGURE11. 1 5 -- chapter FIGURE11. 1 6 -- chapter FIGURE11. 1 7 -- part Appendices -- chapter CONTENTS ————————— A – EQUIPMENT ————————— -- chapter Appendix A -- EQUIPMENT PRACTICAL PRECAUTIONS -- chapter A4 ELECTROPHORESIS OF PCR PRODUCTS IN AGAROSE GEL -- chapter Appendix B -- REAGENTS WATER -- chapter B4 FIXATIVES -- chapter B8 MOUNTING MEDIA -- Aqueous.