Male Infertility Laboratory Investigation: A Critical Analysis of the 2010 World Health Organization Semen Analysis Guidelines -- Intracytoplasmic Injection with Suboptimal Spermatozoa -- Novel Sperm Tests and Their Importance -- Sperm Selection Based on Surface Electrical Charge -- Microfluidics for Sperm Selection -- Sperm Binding to Zona Pellucida, Hyaluronic Acid Binding Assay and PICSI -- Non-Apoptopic Sperm Selection -- Motile Sperm Organelle Morphology Examination (MSOME) -- Setup of Micromanipulator for Sperm Selection and Injection for IMSI -- Configuring the Microscope for Intracytoplasmic Morphology-Selected Sperm Injection (IMSI) -- The Technical Background of Advanced IMSI Systems -- Sperm Vacuoles: Origin and Implications -- MSOME: Conventional Semen Analysis, Sperm Manipulation, and Cryopreservation -- MSOME and Sperm Chromatin Status -- MSOME and Sperm DNA Integrity: Biological and Clinical Considerations -- MSOME and Sperm Chromosomal Constitution -- Intracytoplasmic Morphologically Selected Sperm Injection (IMSI): Indications and Clinical Results -- Genomic and Proteomic Approaches in the Diagnosis of Male Infertility.