Preface -- 1. Mutual influence of cholesterol and PI(4,5)P2 on their levels -- Chapter 1. Mechanism of the regulation of plasma cholesterol levels by PI(4,5)P2 -- Chapter 2. Effect of cholesterol depletion on ciliary PI(4,5)P2 levels -- Chapter 3. Impact of cholesterol on PI(4,5)P2 levels in the brain -- Chapter 4. Alterations in PIP2 levels in the intracellular cholesterol trafficking disorder NPC1 -- Chapter 5. Targeting cholesterol release and PI(4,5)P2 redistribution by the oral drug Miltefosine -- 2. Cross-talk between cholesterol and PI(4,5)P2 as a factor affecting protein function -- A. Plasma membrane ion channels -- Chapter 6. From cross-talk to synergism: the combined effect of cholesterol and PI(4,5)P2 on inwardly rectifying potassium channels -- Chapter 7. Link between lysosomal cholesterol and PI(4,5)P2 in the regulation of the voltage gated KCNQ2/3 potassium channel -- Chapter 8. Cholesterol and PI(4,5)P2 binding to a ciliary membrane transient receptor potential channel -- Chapter 9. Regulation of ell by cholesterol and PI(4,5)P2 -- Chapter 10. Cholesterol stimulates the epithelial sodium channel in a PI(4,5)P2-dependent manner -- Chapter 11. Roles of cholesterol and PI(4,5)P2 in store operated calcium entry -- Chapter 12. Roles of cholesterol and PI(4,5)P2 levels in the modulation of the calcium activated chloride channel TMEM16A -- B. Lipid transport proteins--Chapter 13. PI(4,5)P2 facilitates ORP1-mediated cholesterol transport by promoting membrane targeting and cholesterol extraction -- Chapter 14. Role of PI(4,5)P2 binding in ORP2-mediated cholesterol distribution -- Chapter 15. PI(4,5)P2-dependent transport of cholesterol from lysosomes to peroxisomes at lysosomeperoxisome contact sites -- 3. Cellular processes at the intersection of cholesterol and PI(4,5)P2 involvement -- Chapter 16. The insertion of the N-terminal domain of Gasdermin-D is increased byPI(4,5)P2 and decreased by cholesterol -- Chapter 17. Cholesterol promotes the cooperative interaction of formins with PI(4,5)P2 to initiate actin nucleation -- Chapter 18. Cooperation between cholesterol and PI(4,5)P2 in the regulation of Engrailed-2 homeoprotein intercellular trafficking -- Chapter 19. Cholesterol and PI(4,5)P2 as a membrane nanoplatform for HIV assembly -- Chapter 20. Role of Cholesterol and PI(4,5)P2 in the localization of the HIV-1 structural polyprotein Gag to the plasma membrane.