Part I. Introduction -- An Introduction to Reservoir Ecotoxicology -- Part II. Environmental Characteristics in Reservoir Ecosystems -- Characterization of Reservoir Ecosystem -- Main Sources and Distribution of Pollutants in the Reservoir -- Migration and Transformation of Pollutants in the Reservoir -- Harmful Algal Bloom in the Reservoir -- Microplastics Pollution in the Reservoir: Occurrence, Extraction, and Characterization -- Distribution of Microorganisms in the Reservoir -- Part III. Study Methods of Ecotoxicology in the Reservoir -- Ecotoxicity Test Methods of Primary Producers in the Reservoir -- Ecotoxicology Methods of Reservoir Water Using Invertebrates -- Ecotoxicology Methods of Reservoir Water Using Fish -- Ecotoxicology Methodology of Sediment Toxicity in the Reservoir -- Mesocosm Study in the Reservoir Ecosystem -- Part IV. Ecotoxicological Effects and Mechanism of Pollutants in the Reservoir -- Molecular Toxicity Mechanism of Heavy Metals in the Reservoir -- Molecular Toxicity Mechanism of Persistent Organic Pollutants (Pops) in the Reservoir -- Molecular Toxicity Mechanism of Microplastics in the Reservoir -- Molecular Toxicity Mechanism of Plasticizers in the Reservoir -- Molecular Toxicity Mechanism of PPCPs in the Reservoir -- Adverse Outcome Pathways (AOPs) of Pollutants in the Reservoir -- Invasive Alien Species Problem in the Reservoir -- Part V. Conclusions -- Final Thoughts and Concluding Remarks.