Preface -- Part 1 Basics of Genome Editing -- 1 Genome Editing Using Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) (Hiroshi Ochiai and Takashi Yamamoto)-. 2 CRISPR/Cas9: The Leading Edge of Genome Editing Technology (Tetsushi Sakuma and Takashi Yamamoto)-. Part 2 Genome Editing in Cultured Cells and Various Organisms -- 3 Editing in Cultured Human Cells: From Cell Lines to iPS Cells (Hongmei Lisa Li and Akitsu Hotta)-. 4 Genome Editing in Nematode (Takuma Sugi)-. 5 Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools (Takaaki Daimon)-. 6 Genome Editing in Sea Urchin (Naoaki Sakamoto)-. 7 Genome Editing in Ascidians (Nicholas Treen and Yasunori Sasakura)-. 8 Genome Editing in Zebrafish and Medaka (Atsuo Kawahara, Taijiro Yabe, Satoshi Ansai, Shinji Takada, and Masato Kinoshita)-. 9 Genome Editing Using Site-Specific Nucleases in Amphibians (Ken-ichi T. Suzuki and Toshinori Hayashi)-. 10 Genome Editing in Mice Using CRISPR/Cas  (Samantha AM Young, Mark Baker, and Masahito Ikawa)-. 11 Genome Editing in Mice Using TALENs (Tomomi Aida)-. 12 Engineered Nucleases Lead to Genome Editing Revolution in Rats (Kazuto Yoshimi, Takehito Kaneko, Birger Voigt, and Tomoji Mashimo)-. 13 Genome Editing in Higher Plants (Yuriko Osakabe and Keishi Osakabe).