part Theme 1 Fundamental Aspects -- chapter 1 Principles of Cryobiology* / chapter 2 The Water to Ice Transition: Implications for Living Cells / part Theme 2 Life and Death at Low Temperatures -- chapter 3 Life in the Polar Terrestrial Environment with a Focus on Algae and Cyanobacteria* / chapter 4 Microbial Life in Permafrost: Extended Times in Extreme Conditions / chapter 5 Adaptation of Higher Plants to Freezing / chapter 6 Oxidative Stress in the Frozen Plant: A Free Radical Point of View / chapter 7 Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance: The Wood Frog / part Theme 3 Freezing and Banking of Living Resources -- chapter 8 Preservation of Fungi and Yeasts / chapter 9 Cryoconserving Algal and Plant Diversity: Historical Perspectives and Future Challenges / chapter 10 Plant Cryopreservation / chapter 11 The Early History of Gamete Cryobiology / chapter 12 Cryobiology of Gametes and the Breeding of Domestic Animals / chapter 13 Cryopreservation as a Supporting Measure in Species Conservation: Not the Frozen Zoo! / chapter 14 Cryopreservation of Gametes and Embryos of Aquatic Species / chapter 15 Fundamental Issues for Cell-Line Banks in Biotechnology and Regulatory Affairs / part Theme 4 Medical Applications -- chapter 16 Mechanisms of Injury Caused by in Vivo Freezing / chapter 17 Cryopreservation in Transfusion Medicine and Hematology / chapter 18 Cryopreservation of Human Gametes and Embryos / chapter 19 The Scientific Basis for Tissue Banking / chapter 20 Engineering Desiccation Tolerance in Mammalian Cells: Tools and Techniques / chapter 21 Stabilization of Cells during Freeze-Drying: The Trehalose Myth / chapter 22 Vitrification in Tissue Preservation: New Developments / part Theme 5 The Future of Cryobiology -- chapter 23 The Future of Cryobiology