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Making and using antibodies : a practical handbook

Howard, Gary C.

Making and using antibodies : a practical handbook

Howard, Gary C.

9781439869109

Second edition.

1 online resource (xiv, 444 pages)

ch. 1. Antibodies / Matthew R. Kaser and Gary C. Howard -- ch. 2. Antigens and adjuvants / Paul Algate, Jory Baldridge, and Sally P. Mossman -- ch. 3. Production of polyclonal antibodies / Lon V. Kendall -- ch. 4. Purification and characterization of antibodies / Joseph P. Chandler -- ch. 6. Production of monoclonal antibodies / Kathleen C.F. Sheehan -- ch. 6. Quantitative production of monoclonal antibodies / David A. Fox and Elizabeth M. Smith -- ch. 7. Making antibodies in bacteria / Frederic A. Fellouse and Sachdev S. Sidhu -- ch. 8. In vitro selection of aptamers / Tianjiao Wang, Xiangyu Cong, and Marit Nilsen-Hamilton -- ch. 9. Chemical and proteolytic modification of antibodies / George P. Smith -- ch. 10. Western blots and other applications / Lee Bendickson and Marit Nilsen-Hamilton -- ch. 11. Immunohistochemical methods / Jose A. Ramos-Vara -- ch. 12. Immunoelectron microscopy / Sara E. Miller and David N. Howell -- ch. 13. Flow cytometry / Steven B. McClellan -- ch. 14. ELISAs / John Chen and Gary C. Howard -- ch. 15. Humanization of antibodies / Juan Carlos Almagro, Sreekumar Kodangattil, and Jian Li -- ch. 16. Antibodies in the future : challenges and opportunities / Gary C. Howard and Matthew R. Kaser.

This second edition provides guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included--Provided by publisher.

Immunoglobulins -- Handbooks, manuals, etc.

Howard, Gary C.

Kaser, Matthew R.

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