1. Methods for conducting connected culture experiments using the Quasi-Vivo chambers / Tommaso Sbrana and Arti Ahluwalia -- 2. The use of in vitro 3D cell models of human airway epithelia (MucilAir) in inhalation toxicity / Samual Constant, Ludovic Wiszniewski, and Song Huang -- 3. Cultivation of human hepatocytes in the Quasi-Vivo system : from isolation and seeding to quantification of xenobiotic-metabolizing enzyme expression and activity / Cedric Duret, Bruna Vinci, Tommaso Sbrana, Sylvie Klieber, Patrick Maurel, Martine Daujat-Chavanieu, and Arti Ahluwalia -- 4. Generation of patient-specific cardiac patches by human cardiac progenitor cells and 3D scaffolds / Giancarlo Forte -- 5. In vitro risk assessment of nanoparticles / Birgit K. Gaiser, Julia Susewind, Nadia Ucciferri, Eva-Maria Collnot, Arti Ahluwalia, and Vicki Stone -- 6. Utilizing nanosensor-incorporated scaffolds in the development of a 3D lung model / Helen C. Harrington, Felicity R.A.J. Rose, Amir M. Ghaemmaghami, and Jonathan W. Aylott -- 7. Development of a paracrine-signaling model for detecting irritancy using human keratinocytes and fibroblasts / Jirapat Chunthapong, Sheila MacNeil, and John W. Haycock -- 8. Advanced in vitro models of the intestinal mucosa for drug delivery studies / Eva-Maria Collnot, Julia Susewind and Claus-Michael Lehr -- 9. An in vitro NHBE model of the human bronchial epithelium / Zoe Prytherch and Kelly BeruBe -- 10. Method for simple and routine three-dimensional cell culture / Eleanor Knight and Stefan Przyborski.