chapter 1 Cell Culture Technology�An Overview / chapter 2 Recombinant DNA Technology and Cell Line Development / chapter 3 Medium Development / chapter 4 Cell Metabolism / chapter 5 Protein Glycosylation / chapter 6 Cell Culture Bioreactors / chapter 7 Aeration, Mixing and Hydrodynamics in Bioreactors / chapter 8 Instrumentation and Process Control / chapter 9 Cell Culture Kinetics and Modeling -- chapter 10 Fed-Batch Cultivation of Mammalian Cells for the Production of Recombinant Proteins / chapter 11 Optimization of High Cell Density Perfusion Bioreactors / chapter 12 Cell Separation and Product Capture -- chapter 13 Downstream Processing / chapter 14 Formulation, Filling and Packaging / chapter 15 Validation of Cell Culture-Based Processes and Qualification of Associated Equipment and Facility / chapter 16 Facility Design / chapter 17 Production of Proteins by Transient Expression / chapter 18 Principles and Applications of the Insect Cell-Baculovirus Expression Vector System / chapter 19 Advances in Adult Stem Cell Culture / chapter 20 Ex Vivo Culture of Hematopoietic and Mesenchymal Stem Cells for Tissue Engineering and Cell-Based Therapies