Front Matter -- Introduction to Optics / Rainer Heintzmann, Ulrich Kubitscheck -- Principles of Light Microscopy / Ulrich Kubitscheck -- Fluorescence Microscopy / Jurek W Dobrucki, Ulrich Kubitscheck -- Fluorescence Labeling / Gerd Ulrich Nienhaus, Karin Nienhaus -- Confocal Microscopy / Nikolaus Naredi-Rainer, Jens Prescher, Achim Hartschuh, Don C Lamb -- Two-Photon Excitation Microscopy for Three-Dimensional Imaging of Living Intact Tissues / David W Piston -- Light Sheet Microscopy / Gopi Shah, Michael Weber, Jan Huisken -- Localization-Based Super-Resolution Microscopy / Markus Sauer, Mike Heilemann -- Super-Resolution Microscopy: Interference and Pattern Techniques / Udo Birk, Gerrit Best, Roman Amberger, Christoph Cremer -- STED Microscopy / Travis J Gould, Lena K Schroeder, Patrina A Pellett, Joerg Bewersdorf -- Fluorescence Photobleaching Techniques / Reiner Peters -- Single-Molecule Microscopy in the Life Sciences / Markus Axmann, Josef Madl, Gerhard J Schütz -- Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging / Fred S Wouters -- What Exactly is a Digital Image? -- Practical Guide to Optical Alignment -- Index.