Principles of Gradient Elution. Aspects of Gradient Optimization / Stavros Kromidas -- Instrumental Influences on the Quality and Performance of Gradient Methods and Their Transfer Between Different HPLC Devices / Frank Steiner -- Optimization of a Reversed-Phase Gradient Separation Using EXCEL / Hans-Joachim Kuss -- Specifics of the Gradient in Different Elution Modes. Gradient Elution of Ionic Compounds / Joachim Weiss -- The Gradient in Biochromatography / Oliver Genz -- Specifications of Gradients in Hydrophilic Interaction Liquid Chromatography (HILIC) / Thomas Letzel -- Specifications of Gradients in Supercritical Fluid Chromatography / Stefan Bieber, Thomas Letzel -- Aspects of Gradient Elution in LC'MS Analysis / Markus M Martin -- Additional Tools for Method Development: Flow and Temperature Gradients / Egidijus Machtejevas -- Index