Optimization Strategies for Different Modes and Uses of HPLC. 2D-HPLC- Method Development for Successful Separations / Dwight R Stoll, PhD -- Do you HILIC? With Mass Spectrometry? Then do it Systematically / Thomas Letzel -- Optimization Strategies in LC-MS Method Development / Markus M Martin -- Chromatographic Strategies for the Successful Characterization of Protein Biopharmaceuticals / Szabolcs Fekete, Valentina D'Atri, Davy Guillarme -- Optimization Strategies in HPLC for the Separation of Biomolecules / Lisa Strasser, Florian Fossl, Jonathan Bones -- Optimization Strategies in Packed-Column Supercritical Fluid Chromatography (SFC) / Caroline West -- Strategies for Enantioselective (Chiral) Separations / Markus Juza -- Optimization Strategies Based on the Structure of the Analytes / Christoph A Fleckenstein -- Optimization Opportunities in a Regulated Environment / Stavros Kromidas -- Computer-aided Optimization. Strategy for Automated Development of Reversed-Phase HPLC Methods for Domain-Specific Characterization of Monoclonal Antibodies / Jennifer La, Mark Condina, Leexin Chong, Craig Kyngdon, Matthias Zimmermann, Sergey Galushko -- Fusion QbD Software Implementation of APLM Best Practices for Analytical Method Development, Validation, and Transfer / Richard Verseput -- Current Challenges for HPLC Users in Industry. Modern HPLC Method Development / Stefan Lamotte -- Optimization Strategies in HPLC from the Perspective of an Industrial Service Provider / Juri Leonhardt, Michael Haustein -- Optimization Strategies in HPLC from the Perspective of a Service Provider -- The UNTIE Process of the CUP Laboratories / Dirk Freitag-Stechl, Melanie Janich -- Optimization Strategies in HPLC / Bernard Burn -- Current Challenges for HPLC Equipment Suppliers. Optimization Strategies with your HPLC- Agilent Technologies / Jens Trafkowski -- To Empower the Customer -- Optimization Through Individualization / Kristin Folmert, Kathryn Monks -- (U)HPLC Basics and Beyond / Gesa Schad, Brigitte Bollig, Kyoko Watanabe -- Addressing Analytical Challenges in a Modern HPLC Laboratory / Frank Steiner, Soo Hyun Park -- Systematic Method Development with an Analytical quality-by-Design Approach Supported by Fusion QbD and UPLC-MS / Falk-Thilo Ferse, Detlev Kurth, Tran N Pham, Fadi L Alkhateeb, Paul Rainville.