Ch. 1. A brief history of enzymology -- Ch. 2. Chemical bonds and reactions in biochemistry -- Ch. 3. Structural Components of Enzymes -- Ch. 4. Protein - ligand binding equilibria -- Ch. 5. Kinetics of single-substrate enzyme reactions -- Ch. 6. Chemical mechanisms in enzyme catalysis -- Ch. 7. Experimental measures of enzyme activity -- Ch. 8. Reversible inhibitors -- Ch. 9. Tight binding inhibitors -- Ch. 10. Time-dependent inhibition -- Ch. 11. Enzyme reactions with multiple substrates -- Ch. 12. Cooperativity in enzyme catalysis -- App. I. Suppliers of reagents and equipment for enzyme studies -- App. II. Useful computer software and web sites for enzyme studies.