chapter 1. Metabolomics in a nutshell / Sastia Prama Putri and Eiichiro Fukusaki -- chapter 2. Design of metabolomics experiment / Sastia Prama Putri, Fumio Matsuda, and Takeshi Bamba -- chapter 3. Sample preparation / Yoshihiro Izumi, Walter A. Lavina, and Sastia Prama Putri -- chapter 4. Gas chromatography/mass spectrometry analysis : nontargeted metabolomics based on scan mode analysis / Hiroshi Tsugawa and Arjen Lommen -- chapter 5. LC/QqQ/MS analysis : widely targeted metabolomics on the basis of multiple reaction monitoring / Fumio Matsuda and Hiroshi Tsugawa -- chapter 6. Statistical analysis / Hiroshi Tsugawa and Takeshi Bamba -- chapter 7. Case studies / Walter A. Lavina, Yusuke Fujieda, Udi Jumhawan, Sastia Prama Putri, and Eiichiro Fukusaki.