1. Introduction -- 2. What NEWGARDEN does -- 3. Analyzing genetic diversity in small, isolated, and developing populations -- 4. Input -- 5. Output : what NEWGARDEN tallies, calculates, and reports -- 6. Using NEWGARDEN -- 7. Founder number and allelic diversity -- 8. Population genetics edge effects -- 9. Spatial arrangement of founders -- 10. The rate of reproduction -- 11. Truly random mating and selfing rate -- 12. Dispersal -- 13. The 50/500-100-1000-5000 Ne rules, actual population size, and loss of diversity to random drift -- 14. Dioecy -- 15. Perennials -- 16. Actual data versus NEWGARDEN : American chestnut -- 17. Corridors -- 18. Conclusions and discussion.