chapter 1. Advances in aerosol-based detectors / Ryan D. Cohen and Yong Liu -- chapter 2. Integration of analytical techniques for protein biomarker assays / Jennifer L. Colangelo -- chapter 3. Multilinear gradient elution optimization in liquid chromatography / P. Nikitas, A. Pappa-Louisi, and Ch. Zisi -- chapter 4. Analytical separation of enantiomers by gas chromatography on chiral stationary phases / Volker Schurig and Markus Juza -- chapter 5. Analysis of dynamic phenomena in liquid chromatographic systems with reactions in the mobile phase / Lei Ling and Nien-Hwa Linda Wang.