chapter 1 Appendix -- chapter 2 Optical Contrasting Techniques -- chapter 3 Fluorescence and Fluorescence Microscopy -- chapter 4 Image Capture -- chapter 5 The Scanning Optical Microscope -- chapter 6 Contrast -- chapter 7 Geometrical Aberrations -- chapter 8 Multiphoton Microscopy -- chapter 9 Tandem-Scanning (Spinning Disk) Microscopes -- chapter 10 Deconvolution and Image Processing -- chapter 11 Surfaces: Two-and-a-Half Dimensions -- chapter 12 Structure and Properties of GFP -- chapter 13 Immunolabeling -- chapter 14 Fluorescence Intensity Measurements -- chapter 15 Fluorescence Lifetime -- chapter 16 Near-Field Microscopy.