chapter 1 Analysis of Canonical Wnt Signaling in Xenopus Embryos -- chapter 2 How to Assay Non-Canonical Wnt Signaling: A Critical Analysis -- chapter 3 Regulation of TGF- Family Activity by Proprotein Processing -- chapter 4 Analysis of MAP Kinase Pathways in Vertebrate Development -- chapter 5 Analysis of Retinoid Signaling in Embryos -- chapter 6 Wnt Signaling via the Rho Family of GTPases during Embryonic Development -- chapter 7 Phosphospecific Antibodies as Tools for the Study of Signal Transduction during Development -- part Section II: Ionic Signals -- chapter 8 Dynamic Analysis of Calcium Signaling in Animal Development -- chapter 9 Strategies and Techniques for Investigation of Biophysical Signals in Patterning -- part Section III: Transcriptional Regulation of Target Genes -- chapter 10 Expression Profiling in Xenopus Embryos -- chapter 11 Chromatin Immunoprecipitation for In Vivo Studies of Transcriptional Regulation during Development -- part Section IV: Emerging Strategies for the Analysis of Signaling in Development -- chapter 12 Chemical Biology in Zebrafish Vascular Development -- chapter 13 Investigating Gastrulation -- chapter 14 Bringing Small Molecule Regulation of Protein Activity to Developmental Systems -- chapter 15 Systems Analysis of Signaling Pathways.