chapter 1 Protein Kinases in Drug Discovery: Rationale, Success, and Challenge -- chapter 2 An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development -- chapter 3 Time-Resolved Fluorescence Based Assays for Kinases -- chapter 4 Development of High-Throughput Screening Assays in Scintillating Microplates (FlashPlates) to Identify Inhibitors of Kinase Activity -- chapter 5 Development of High-Throughput Screening Assays for Kinase Drug Targets Using AlphaScreen Technology -- chapter 6 Homogeneous High-Throughput Screening (HTS) Assays for Serine/Threonine Kinases Using -Galactosidase Enzyme Fragment Complementation -- chapter 7 IMAP Assays for Protein Kinases -- chapter 8 A Homogeneous, Luminescent, High-Throughput, Versatile Assay for a Wide Range of Kinases -- chapter 9 Proteases as Drug Targets -- chapter 10 A Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays -- chapter 11 Scintillation Proximity Assay (SPA) Receptor Binding Assays -- chapter 12 Radioligand Binding Filtration Assay: Full Automation -- chapter 13 Nuclear Receptors as Drug Targets -- chapter 14 Nuclear Receptor Scintillation Proximity Assays -- chapter 15 Homogeneous Assay Development for Nuclear Receptor Using AlphaScreen Technology / chapter 16 Development of Nuclear Receptor Homogeneous Assay Using the Lance Technology / chapter 17 The Emerging Role of Cell-Based Assays in Drug Discovery / chapter 18 The Preparation of Cells for High-Content Screening / chapter 19 The Evolution of cAMP Assays / chapter 20 A Homogeneous, Fluorescent Polarization Assay for Inositol 1,4,5-Trisphosphate (Ins P3) / chapter 21 Scintillation Proximity Assay of Inositol Phosphates / chapter 22 Measuring Calcium Mobilization with G -Coupled GPCRs Using the Fluormetric Imaging Plate Reader (FLIPR) / chapter 23 Development of FLIPR-Based HTS Assay for G;-Coupled GPCRs / chapter 24 Aurora Assays / chapter 25 Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters / chapter 26 Reporter Gene Assays for Drug Discovery / chapter 27 mRNA Detection from Cells Using Quantigene Branched DNA Technology. Lisa Minor -- chapter 28 Homogeneous Multiwell Assays for Measuring Cell Viabiltiy, Cytotoxicity, and Apoptosis / chapter 29 Development of Image-Based Assays for Drug Discovery / chapter 30 GPCR Internalization Measured by Image Analysis / chapter 31 TRANSFLUOR Provides a Universal Cell-Based Assay for Screening G-Protein-Coupled Receptors