Başlık:
Intracellular thermometry with fluorescent molecular thermometers
Yazar:
Uchiyama, Seiichi, author.
ISBN:
9783527836840
9783527836864
Fiziksel Tanımlama:
1 online resource
İçerik:
Intro -- Intracellular Thermometry with Fluorescent Molecular Thermometers -- Preface -- Contents -- 1 Temperature for Living Things -- 1.1 Temperature of Individuals -- 1.2 Responses to Temperature Variation at the Cellular Level -- 1.3 Significance of Intracellular Thermometry -- References -- 2 Fluorescent Molecular Thermometers -- 2.1 The Basics of Fluorescence -- 2.2 Responses of Fluorescent Molecular Thermometers -- 2.3 Small Organic Molecules Involving Intersystem Crossing -- 2.4 Small Organic Molecules with a Rotating Substituent Group -- 2.5 Reactive Small Organic Molecules
2.6 Viscosity-Sensitive Small Organic Molecules -- 2.7 Other Small Organic Molecules -- 2.8 Organometallic Complexes -- 2.9 Excimers and Exciplexes -- 2.10 Host-guest Interactions -- 2.11 Synthetic Polymers -- 2.12 Nucleic Acids (DNA and RNA) -- 2.13 Peptides -- 2.14 Fluorescent Proteins -- 2.15 Non-covalent Systems Based on Thermo-responsive Self-assembly and an Environment-sensitive Fluorophore -- 2.16 Inorganic Nanomaterials -- 2.17 Hybrid Nanomaterials -- References -- 3 Intracellular Thermometry with Fluorescent Molecular Thermometers -- 3.1 Early Attempts for Cellular Thermometry
3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell -- 3.3 Cytotoxicity Assessment -- 3.4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers -- 3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers -- 3.6 Intracellular Thermometry of Brown Adipocytes -- 3.7 Application of Intracellular Thermometry in Various Biological Fields -- References -- 4 Cellular Thermometry Based on Non-fluorometric Principles -- 4.1 Infrared Thermometry -- 4.2 Photoacoustic Thermometry -- 4.3 Raman Thermometry
4.4 Use of Transmission Spectroscopy -- 4.5 Thermocouple -- 4.6 Resonant Thermal Sensor -- 4.7 Bimaterial Microcantilever -- 4.8 Thermistor -- References -- 5 Reliability Issue in Intracellular Thermometry -- 5.1 Sensitivity and Temperature Resolution -- 5.2 Functional Independency of Fluorescent Molecular Thermometers -- 5.3 Preparation of a Calibration Curve of Fluorescent Molecular Thermometers -- 5.4 Objection to Endogenous Thermogenesis -- 5.5 Possible Artifacts in Near-infrared Luminescent Thermometry and Proposal for Reliable Thermometry -- References
6 Applications of Intracellular Thermometry -- 6.1 Creation of New Biological Concepts -- 6.2 In vivo Temperature Measurements -- 6.3 Thermal Medicine at a Single-cell Level -- 6.4 Utilization of Nanoheater/Fluorescent Thermometer Hybrids in Biotechnology -- 6.5 Accurate Measurements of Absolute Intracellular Temperature -- 6.6 Simultaneous Monitoring of Intracellular Temperature and the Concentration of a Chemical Species Related to a Temperature Variation Inside a Living Cell -- 6.7 Intracellular Thermometry of Plant Cells -- References
Özet:
Intracellular Thermometry with Fluorescent Molecular Thermometers Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.
Notlar:
John Wiley and Sons
Tür:
Elektronik Erişim:
https://onlinelibrary.wiley.com/doi/book/10.1002/9783527836840Kopya:
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