The two-volume set LNCS 14499 and 14500 constitutes the proceedings of the 25th International Conference on Verification, Model Checking, and Abstract Interpretation, VMCAI 2024, which took place in London, Ontario, Canada, in January 2024. The 30 full papers presented in the proceedings were carefully reviewed and selected from 74 submissions. They were organized in topical sections as follows: Part I: Abstract interpretation; infinite-state systems; model checking and synthesis; SAT, SMT, and automated reasoning; Part II: Concurrency; neural networks; probabilistic and quantum programs; program and system verification; runtime verification; security and privacy.